Intelligent quantum dot-based lighting system reproduces daylight more accurately

Dual plasmid editing system improves DNA digital storage capabilities

DNA-based information is a new interdisciplinary field linking information technology and biotechnology. The field hopes to meet the huge need for long-term data storage by using DNA as a means of storing information. Although DNA promises robust stability, high storage density and low maintenance cost, researchers are having problems accurately rewriting the digital information encoded in the DNA sequence.

In general, the DNA data storage technology has two modes, i.e. “in vitro hard disk mode” and “in vivo CD mode”. The primary advantage of the in vivo setting is the low cost and reliable replication of chromosomal DNA by cell proliferation. Due to this characteristic, it can be used to spread data copies quickly and at low cost. Since the DNA sequences encoding some information contain a large number of repeats and the appearance of homopolymers, this information can only be “written” and “read”, but not precisely “rewritten”.

To solve the rewriting problem, Dr. LIU Kai from the Department of Chemistry, Tsinghua University, Prof. LI Jingjing of the Changchun Institute of Applied Chemistry (CIAC) of the Chinese Academy of Sciences, and Prof. Qin Dong from Zhejiang University led a research team that recently developed a dual-plasmid editing system to precisely process digital information in a microbial vector. Their findings were published in Science Advances.

The researchers created an in vivo binary plasmid system using a rationally designed coding algorithm and information editing tool. This two-plasmid system is suitable for storing, reading and rewriting various types of information, including text, codebooks, and images. It fully explores the coding ability of DNA sequences without the need for any addressing pointers or backup sequences. It is also compatible with various types of encryption algorithms, enabling high encryption efficiency. For example, the coding efficiency of the current system is up to 4.0 bits per nucleotide.

To achieve high efficiency as well as reliability in rewriting the complex information stored in exogenous DNA sequences in vivo, a variety of CRISPR-related (Cas) and recombinase-related proteins have been used. The tools were directed by CRISPR RNA (crRNA) corresponding to the cleavage of the target locus in the DNA sequence so that the selected information could be processed and rewritten. Due to the high specificity between the complementary pairs of DNA molecules, the informative DNA sequences were precisely reconstructed by recombinase to encode the new information. Due to the optimization of crRNA sequencing, the information rewriting tool has become highly adaptable to complex information, resulting in a rewriting reliability of up to 94%, which is comparable to current gene editing systems.

The double plasmid system can serve as a global platform for DNA-based information rewriting in vivo, thus presenting a new strategy for target-specific information processing and rewriting of large and complex data at the molecular level.

Professor A.D. LIU.

/General release. This material from the original organization/author(s) may be of a point in time nature, and is edited for clarity, style and length. The opinions and opinions expressed are those of the author(s), view them in full here.

Leave a Comment

Your email address will not be published.